P‐75 Development of an in vitro test to evaluate cerumen‐dissolving properties of veterinary ear cleansing solutions
Identifieur interne : 000581 ( France/Analysis ); précédent : 000580; suivant : 000582P‐75 Development of an in vitro test to evaluate cerumen‐dissolving properties of veterinary ear cleansing solutions
Auteurs : F. Nielloud [France] ; C. A. Reme [France] ; R. Fortune [France] ; J. P. Laget [France] ; G. Mestres [France] ; H. Gatto [France]Source :
- Veterinary Dermatology [ 0959-4493 ] ; 2004-08.
Abstract
This study aimed to evaluate objectively the cleansing properties of four veterinary ear care solutions. An in vitro test was developed, based on dissolution of synthetic dog cerumen in test tubes. Artificial cerumen was produced, based on literature data, with a melting point and consistency very close to natural canine cerumen. One millilitre of test solution was poured into haemolysis tubes containing 100 mg of synthetic cerumen. Contact time was 10 min at 37°C. Moderate agitation was then applied for 10 s to mimic in vivo application. Solutions were vacuum‐filtered on filter paper and cerumen remaining in the tube or on the paper was weighed after drying. The experiment was repeated five times for each formulation. One‐way ANOVA was performed to detect differences between formulations. In order to validate the test, measurement of the solution wetting power was performed in parallel using the reference ISO 8022 method. Mean quantities of cerumen dissolved were 4.7, 6.5, 7.7, 14.7 and 23.2 mg for Cerulane®, Otolane®, distilled water (control), Otoclean® and Physiological Ear Cleanser®, respectively. The latter was the only solution that demonstrated greater ability for cerumen dissolution as compared to water (P < 0.05). Macroscopically, Physiological Ear Cleanser® solution became white and opaque in test tubes, reflecting the formation of an emulsion. Other solutions remained clear or only partially turbid. This cerumen dissolution test proved a practical and valuable model to assess the cleansing power of ear solutions before in vivo trials. Funding: Virbac SA.
Url:
DOI: 10.1111/j.1365-3164.2004.00414_75.x
Affiliations:
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Development of an <hi rend="italic">in vitro</hi> test to evaluate cerumen‐dissolving properties of veterinary ear cleansing solutions</title><author><name sortKey="Nielloud, F" sort="Nielloud, F" uniqKey="Nielloud F" first="F." last="Nielloud">F. Nielloud</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Industrial Pharmaceutical Technique, Faculty of Pharmacy, Montpellier, France; Virbac SA, Carros</wicri:regionArea><wicri:noRegion>Carros</wicri:noRegion><wicri:noRegion>Carros</wicri:noRegion></affiliation></author><author><name sortKey="Reme, C A" sort="Reme, C A" uniqKey="Reme C" first="C. A." last="Reme">C. A. Reme</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Industrial Pharmaceutical Technique, Faculty of Pharmacy, Montpellier, France; Virbac SA, Carros</wicri:regionArea><wicri:noRegion>Carros</wicri:noRegion><wicri:noRegion>Carros</wicri:noRegion></affiliation></author><author><name sortKey="Fortune, R" sort="Fortune, R" uniqKey="Fortune R" first="R." last="Fortune">R. Fortune</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Industrial Pharmaceutical Technique, Faculty of Pharmacy, Montpellier, France; Virbac SA, Carros</wicri:regionArea><wicri:noRegion>Carros</wicri:noRegion><wicri:noRegion>Carros</wicri:noRegion></affiliation></author><author><name sortKey="Laget, J P" sort="Laget, J P" uniqKey="Laget J" first="J. P." last="Laget">J. P. Laget</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Industrial Pharmaceutical Technique, Faculty of Pharmacy, Montpellier, France; Virbac SA, Carros</wicri:regionArea><wicri:noRegion>Carros</wicri:noRegion><wicri:noRegion>Carros</wicri:noRegion></affiliation></author><author><name sortKey="Mestres, G" sort="Mestres, G" uniqKey="Mestres G" first="G." last="Mestres">G. Mestres</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Industrial Pharmaceutical Technique, Faculty of Pharmacy, Montpellier, France; Virbac SA, Carros</wicri:regionArea><wicri:noRegion>Carros</wicri:noRegion><wicri:noRegion>Carros</wicri:noRegion></affiliation></author><author><name sortKey="Gatto, H" sort="Gatto, H" uniqKey="Gatto H" first="H." last="Gatto">H. Gatto</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Industrial Pharmaceutical Technique, Faculty of Pharmacy, Montpellier, France; Virbac SA, Carros</wicri:regionArea><wicri:noRegion>Carros</wicri:noRegion><wicri:noRegion>Carros</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Veterinary Dermatology</title><title level="j" type="alt">VETERINARY DERMATOLOGY</title><idno type="ISSN">0959-4493</idno><idno type="eISSN">1365-3164</idno><imprint><biblScope unit="vol">15</biblScope><biblScope unit="page" from="65">65</biblScope><biblScope unit="page" to="65">65</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2004-08">2004-08</date></imprint><idno type="ISSN">0959-4493</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0959-4493</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study aimed to evaluate objectively the cleansing properties of four veterinary ear care solutions. An in vitro test was developed, based on dissolution of synthetic dog cerumen in test tubes. Artificial cerumen was produced, based on literature data, with a melting point and consistency very close to natural canine cerumen. One millilitre of test solution was poured into haemolysis tubes containing 100 mg of synthetic cerumen. Contact time was 10 min at 37°C. Moderate agitation was then applied for 10 s to mimic in vivo application. Solutions were vacuum‐filtered on filter paper and cerumen remaining in the tube or on the paper was weighed after drying. The experiment was repeated five times for each formulation. One‐way ANOVA was performed to detect differences between formulations. In order to validate the test, measurement of the solution wetting power was performed in parallel using the reference ISO 8022 method. Mean quantities of cerumen dissolved were 4.7, 6.5, 7.7, 14.7 and 23.2 mg for Cerulane®, Otolane®, distilled water (control), Otoclean® and Physiological Ear Cleanser®, respectively. The latter was the only solution that demonstrated greater ability for cerumen dissolution as compared to water (P < 0.05). Macroscopically, Physiological Ear Cleanser® solution became white and opaque in test tubes, reflecting the formation of an emulsion. Other solutions remained clear or only partially turbid. This cerumen dissolution test proved a practical and valuable model to assess the cleansing power of ear solutions before in vivo trials. Funding: Virbac SA.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Nielloud, F" sort="Nielloud, F" uniqKey="Nielloud F" first="F." last="Nielloud">F. Nielloud</name></noRegion><name sortKey="Fortune, R" sort="Fortune, R" uniqKey="Fortune R" first="R." last="Fortune">R. Fortune</name><name sortKey="Gatto, H" sort="Gatto, H" uniqKey="Gatto H" first="H." last="Gatto">H. Gatto</name><name sortKey="Laget, J P" sort="Laget, J P" uniqKey="Laget J" first="J. P." last="Laget">J. P. Laget</name><name sortKey="Mestres, G" sort="Mestres, G" uniqKey="Mestres G" first="G." last="Mestres">G. Mestres</name><name sortKey="Reme, C A" sort="Reme, C A" uniqKey="Reme C" first="C. A." last="Reme">C. A. Reme</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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